Isocitrate Dehydrogenase 1/IDH1 Antibody

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Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-52119] - Analysis of Isocitrate dehydrogenase (aa65-77) in HepG2 lysate (35ug protein in RIPA buffer) using NBP1-52119 at 0.1ug/ml. Primary incubation was 1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Isocitrate Dehydrogenase 1/IDH1 Antibody Summary

Immunogen
Peptide with sequence KKHNVGVKCATIT corresponding to internal region according to NP_005887.2.
Epitope
KKHNVGVKCATIT
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IDH1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Approx. 40+45 kDa band bands observed in lysates of HepG2 and 40 kDa band in mouse and rat liver lysates (calculated MW of 46.7 kDa band according to NP_005887.2). The observed 40 kDa band is consistent with observaton with different antibodies from other sources.
Publications
Read Publication using NBP1-52119.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine, Sheep.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody

  • Cytosolic NADP-isocitrate dehydrogenase
  • EC 1.1.1.42
  • IDCD
  • IDH
  • IDH1
  • IDP
  • IDPC
  • isocitrate dehydrogenase [NADP] cytoplasmic
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • Isocitrate Dehydrogenase 1
  • NADP(+)-specific ICDH
  • NADP-dependent isocitrate dehydrogenase, cytosolic
  • NADP-dependent isocitrate dehydrogenase, peroxisomal
  • Oxalosuccinate decarboxylase
  • PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IDH1
Entrez
Uniprot