Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9)

Images

 
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Isocitrate dehydrogenasemonoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Isocitrate dehydrogenase.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Endometrium tissue.
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Isocitrate dehydrogenase(Right lane) cDNA for 48 hrs and ...read more
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded breast tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded endometrium tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) Summary

Immunogen
Protein expressed in 293T cell transfected with human IDH1 expression vector.
Specificity
This antibody is specific for Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IDH1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Reviewed Applications
Read 1 Review rated 4
using
NBP1-47804 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9)

  • Cytosolic NADP-isocitrate dehydrogenase
  • EC 1.1.1.42
  • IDCD
  • IDH
  • IDH1
  • IDP
  • IDPC
  • isocitrate dehydrogenase [NADP] cytoplasmic
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • Isocitrate Dehydrogenase 1
  • NADP(+)-specific ICDH
  • NADP-dependent isocitrate dehydrogenase, cytosolic
  • NADP-dependent isocitrate dehydrogenase, peroxisomal
  • Oxalosuccinate decarboxylase
  • PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-47804) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-47804:
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Images Ratings Applications Species Date Details
Western Blot Isocitrate Dehydrogenase 1/IDH1 NBP1-47804
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reviewed by:
Hongxia Hu
WB Human 09/12/2012
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Summary

ApplicationWestern Blot
Sample TestedHep3B WCL
SpeciesHuman

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Recent Reviews

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Hongxia Hu
09/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol IDH1
Entrez
Uniprot