IRS2 [p Ser731] Antibody

Product Discontinued

IRS2 [p Ser731] Antibody Summary

• Immunogen: A synthetic peptide corresponding to the epitope ASSPA with a single phosphorylation site Ser 731 of human IRS-2.
• Modification: p Ser731
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 0.01-0.1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.5-2 ug/ml
  • Immunoprecipitation 2-5 ug/ml
  • Western Blot 0.1-1 ug/ml
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile TBS (pH 7.2) with antibody stabilizer
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRS2 [p Ser731] Antibody

• insulin receptor substrate 2
• IRS2
• IRS-2

Background

Insulin Receptor Substrate-2 (IRS-2), a 180kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, and IRS 4. IRS 2, a signaling molecule that mediates effects of insulin, Insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP-dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has a dual function, serving either for a positive or negative modulation of insulin signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRS2 Antibody (NBP1-72215)(1)

Publication using NBP1-72215

• Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD Cell Signaling Technology, Inc, Beverly, Massachusetts 01915, USA. Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101). J Biol Chem. 279(44):45304-7. 2004-10-29 [PMID: 15364919]

Bioinformatics

• Gene Symbol: IRS2
• Entrez:
  • 8660(Human)
  • 384783(Mouse)
  • 29376(Rat)
• Uniprot:
  • Q9Y4H2(Human)