IRS1 [p Ser639] Antibody

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Immunohistochemistry-Paraffin: IRS1 Antibody [NBP1-51441] - IRS1 [p Ser639] Antibody [NBP1-51441] Analyzes of p-IRS-1 (S639) pAb in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51441
    • Availability
      Product Discontinued

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IRS1 [p Ser639] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 639.
Modification
p Ser639
Specificity
p-IRS-1 (S639) pAb detects endogenous levels of IRS-1 protein only when phosphorylated at S639.
Clonality
Polyclonal
Host
Rabbit
Gene
IRS1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Immunohistochemistry (IHC) analyzes of p-IRS-1 (S639) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
132 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRS1 [p Ser639] Antibody

  • HIRS-1
  • insulin receptor substrate 1
  • IRS1
  • IRS-1

Background

Insulin receptor substrate-1 (IRS-1) is a 170-185 kDa substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. Tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while Serine (Ser)/Threonine (Thr) phosphorylation of IRS-1 can either enhance or negate insulin effects. Tyrosines 465, 612, 632, 662, 941 and 989 of IRS-1 resemble YXXM motifs that upon phosphorylation are predicted to bind SH2 domains in the p85 regulatory subunit of PI3K, resulting in activation of p110 catalytic subunit. SHP-2 binding to IRS-1 can occur upon phosphorylation at Tyr 1179 and Tyr 1229. GRB2 binding can occur upon phsophorylation at Tyr 896. Rodent Ser 99 and Thr 502 of IRS-1 are casein kinase II-dependent phosphorylation sites. There is an increase in Ser 636 phosphorylation of IRS-1 in primary skeletal muscle cells from patients with type 2 diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IRS1
Entrez