IRF9 Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and 3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

IRF9 Antibody Summary

Immunogen
E. coli-derived recombinant human IRF9
Ser238-Val393
Accession # Q00978
Specificity
Detects endogenous human and mouse IRF9 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IRF9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publication using
AF5629 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF9 Antibody

  • IFN-alpha-responsive transcription factor subunit
  • interferon regulatory factor 9
  • Interferon-stimulated gene factor 3 gamma
  • interferon-stimulated transcription factor 3, gamma (48kD)
  • interferon-stimulated transcription factor 3, gamma 48kDa
  • IRF9
  • IRF-9
  • ISGF3 p48 subunit
  • ISGF3
  • ISGF3G
  • ISGF3GISGF-3 gamma
  • p48
  • Transcriptional regulator ISGF3 subunit gamma

Background

IRF9 (interferon regulatory factor 9; also ISGF3-gamma ) is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. Following activation of IFNAR by IFN-alpha / beta, IRF9 is acetylated by CBP on Lys81. IRF9 then associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus. Human IRF9 is 393 amino acids (aa) in length. It contains an N-terminal DNA-binding region (aa 11‑112) that contains an NLS (aa 66‑85), plus a STAT-binding domain (aa 200‑393). There are two potential isoform variants. One shows a deletion of aa 218‑331 and 340‑393, while another shows a five aa substitution for aa 217‑393. Over aa 238‑393, human IRF9 shares 79% aa identity with mouse IRF9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF9 Antibody (AF5629)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IP.


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IP
(1)
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Mouse
(1)
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Bioinformatics

Gene Symbol IRF9
Entrez
Uniprot