IRF6 Antibody (503404)

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IRF6 Monoclonal Antibody (Catalog # MAB6114) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF6 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 503404
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IRF6 Antibody (503404) Summary

• Immunogen: E. coli-derived recombinant human IRF6
  Ala176-Gln366
  Accession # O14896
• Specificity: Detects human and mouse IRF6 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF6 Antibody (503404)

• interferon regulatory factor 6
• IRF6
• IRF-6
• LPS
• OFC6
• OFC6PPS
• PIT
• PPS
• Van der Woude syndrome
• VWS
• VWS1

Background

Interferon regulatory factor 6 (IRF6), is a transcription factor that controls the proliferation and differentiation of epidermal and epithelial cells. IRF6 interacts with maspin in normal mammary epithelial cells but is down‑regulated during tumor progression. IRF6 polymorphisms cause Van der Woude and popliteal pterygium syndromes of skin, limb, and craniofacial development that are characterized by cleft lip, cleft palate, or lip pits. IRF6 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within aa 176‑366 of the C‑terminal domain, human IRF6 shares 98% aa sequence identity with mouse and rat IRF6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF6 Antibody (MAB6114)(1)

Publication using MAB6114

• L Huang, Z Jia, Y Shi, Q Du, J Shi, Z Wang, Y Mou, Q Wang, B Zhang, Q Wang, S Ma, H Lin, S Duan, B Yin, Y Lin, Y Wang, D Jiang, F Hao, L Zhang, H Wang, S Jiang, H Xu, C Yang, C Li, J Li, B Shi, Z Yang Genetic factors define CPO and CLO subtypes of nonsyndromicorofacial cleft PLoS Genet., 2019-10-14;15(10):e1008357. 2019-10-14 [PMID: 31609978] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: IRF6
• Uniprot:
  • O14896()