IRF3 Antibody


Western Blot: IRF3 Antibody [NB600-271] - Nuclear extract (25 ug) from HeLa cells. Antibody used at 0.33 ug/ml.
Immunohistochemistry-Paraffin: IRF3 Antibody [NB600-271] - Human testicular seminoma. Antibody used at a dilution of 1:1,000.
Immunohistochemistry-Paraffin: IRF3 Antibody [NB600-271] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-IRF3 used at a dilution of 1:250. Detection: DAB staining using more

Product Details

Reactivity Hu, PrimateSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1.0 mg/ml

Order Details

IRF3 Antibody Summary

A synthetic peptide that maps to a region between residues 100 and 150 of human Interferon Regulatory Factor 3 using the numbering given in Swiss-Prot entry Q14653 (GeneID 3661).
NB600-271 is specific for human IRF3 protein.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100- 1:500
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody works in Immunohistochemistry-Paraffin, Western Blot: and Immunoprecipitation. In some cases, the antibody may be diluted further than indicated.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Alternate Names for IRF3 Antibody

  • interferon regulatory factor 3
  • IRF-3


IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRF3 Antibody (NB600-271) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on IRF3.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol IRF3

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