IRF3 Antibody

Images

 
Western Blot: IRF3 Antibody [NB600-271] - Detection of Human IRF3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: IRF3 Antibody [NB600-271] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-IRF3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: IRF3 Antibody [NB600-271] - Nuclear extract (25 ug) from HeLa cells. Antibody used at 0.33 ug/ml.
Immunohistochemistry-Paraffin: IRF3 Antibody [NB600-271] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-IRF3 used at a dilution of 1:250. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

IRF3 Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 100 and 150 of human Interferon Regulatory Factor 3 using the numbering given in Swiss-Prot entry Q14653 (GeneID 3661).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
HeLa Lysate (NB800-PC9)
IRF3 Lysate (NBL1-12032)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRF3 Antibody

  • interferon regulatory factor 3
  • IRF3
  • IRF-3

Background

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IRF3 Antibody (NB600-271) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IRF3 Antibody (NB600-271)

Discover related pathways, diseases and genes to IRF3 Antibody (NB600-271). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

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Research Areas for IRF3 Antibody (NB600-271)

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Blogs on IRF3.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Bioinformatics

Gene Symbol IRF3
Entrez
Uniprot