IRF3 Antibody (4D4)

Images

 
Western Blot: IRF3 Antibody (4D4) [NBP1-47813] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-IRF3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (4D4) [NBP1-47813] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF3.
Immunohistochemistry-Paraffin: IRF3 Antibody (4D4) [NBP1-47813] - Staining of paraffin-embedded Human pancreas tissue using anti-IRF3 mouse monoclonal antibody.
Flow Cytometry: IRF3 Antibody (4D4) [NBP1-47813] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF3 antibody, and then analyzed by ...read more
Western Blot: IRF3 Antibody (4D4) [NBP1-47813] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

Product Details

Summary
Product Discontinued
View other related IRF3 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-47813
    • Availability
      Product Discontinued

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IRF3 Antibody (4D4) Summary

Immunogen
Protein expressed in 293T cell transfected with human IRF3 expression vector
Specificity
This antibody is specific for Homo sapiens interferon regulatory factor 3 (IRF3).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IRF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IRF3 Antibody (4D4)

  • interferon regulatory factor 3
  • IRF3
  • IRF-3

Background

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF3 Antibody (NBP1-47813) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRF3 Antibody (NBP1-47813) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IRF3 Products

Bioinformatics Tool for IRF3 Antibody (NBP1-47813)

Discover related pathways, diseases and genes to IRF3 Antibody (NBP1-47813). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IRF3 Antibody (NBP1-47813)

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Pathways for IRF3 Antibody (NBP1-47813)

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PTMs for IRF3 Antibody (NBP1-47813)

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Research Areas for IRF3 Antibody (NBP1-47813)

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Blogs on IRF3.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Bioinformatics

Gene Symbol IRF3
Entrez