IRAK4 [p Thr345] Antibody (A8A8)


Western Blot: IRAK4 [p Thr345] Antibody (A8A8) [NBP1-72255] - Alu RNA-induced IRAK1 and IRAK4 phosphorylation (1:500) in human RPE cells. Courtesy from Dr. Hirano.
Western Blot: IRAK4 [p Thr345] Antibody (A8A8) [NBP1-72255] - The 5 x 107 Jurkat cells were stimulated by 50 ng/ml IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and more
Dot Blot: IRAK4 [p Thr345] Antibody (A8A8) [NBP1-72255] - 1 mg peptide was blot onto NC membrane A: IRAK-4(pT345) B: IRAK-4(non phosphorylated) Followed by Mouse anti IRAK-4 (pT345) incubation at a 1:1000 dilution.

Product Details

Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, DB, ELISA, IHC, IHC-P, IP
0.5 mg/ml

Order Details

IRAK4 [p Thr345] Antibody (A8A8) Summary

A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein.
p Thr345
This antibody recognizes IRAK-4(pT345) with a phosphorylated site at Threonine 345. It does not crossreact with non-phosphospecific peptide.
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS saline (pH7.2) containing antibody stabilizer.
No Preservative
0.5 mg/ml
Protein A or G purified


Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
51 kDa

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for IRAK4 [p Thr345] Antibody (A8A8)

  • EC 2.7.11
  • EC
  • interleukin-1 receptor-associated kinase 4
  • IPD1
  • IRAK-4
  • NY-REN-64
  • REN64
  • Renal carcinoma antigen NY-REN-64


IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK familymember necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
Species: Mu
Applications: Flow, ICC
Species: Hu, Mu, Rt, Xp, Ze
Applications: B/N, ELISA, In vitro, In vivo, Func-Inh
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Applications: Func-Inh
Species: Hu, Mu, Rt
Applications: B/N, In vitro
Species: Hu, Ca
Applications: B/N, Flow, ICC/IF, IP, In vitro, Flow-CS, Flow-IC
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC, IF
Species: Hu, Mu, Rt, Ca, Eq, Pm
Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC-P, IP, In vitro, Flow-IC
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: In vitro, In vivo, Func-Inh
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu
Applications: WB
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Bv, Ca
Applications: WB, DB, ELISA, IHC, IHC-P, IP

Publications for IRAK4 Antibody (NBP1-72255) (0)

There are no publications for IRAK4 Antibody (NBP1-72255).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IRAK4 Antibody (NBP1-72255) (0)

There are no reviews for IRAK4 Antibody (NBP1-72255). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IRAK4 Antibody (NBP1-72255) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional IRAK4 [p Thr345] Antibody (A8A8) Products

Related Products by Gene

Bioinformatics Tool for IRAK4 Antibody (NBP1-72255)

Discover related pathways, diseases and genes to IRAK4 Antibody (NBP1-72255). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IRAK4 Antibody (NBP1-72255)

Discover more about diseases related to IRAK4 Antibody (NBP1-72255).

Pathways for IRAK4 Antibody (NBP1-72255)

View related products by pathway.

PTMs for IRAK4 Antibody (NBP1-72255)

Learn more about PTMs related to IRAK4 Antibody (NBP1-72255).

Research Areas for IRAK4 Antibody (NBP1-72255)

Find related products by research area.

Blogs on IRAK4.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol IRAK4

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-72255 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought