IRAK4 [p Thr345] Antibody (A8A8)

Images

 
Western Blot: IRAK4 [p Thr345] Antibody (A8A8) [NBP1-72255] - Alu RNA-induced IRAK1 and IRAK4 phosphorylation (1:500) in human RPE cells. Courtesy from Dr. Hirano.
Western Blot: IRAK4 [p Thr345] Antibody (A8A8) [NBP1-72255] - The 5 x 107 Jurkat cells were stimulated by 50 ng/ml IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and ...read more
Dot Blot: IRAK4 [p Thr345] Antibody (A8A8) [NBP1-72255] - 1 mg peptide was blot onto NC membrane A: IRAK-4(pT345) B: IRAK-4(non phosphorylated) Followed by Mouse anti IRAK-4 (pT345) incubation at a 1:1000 dilution.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-72255
    • Availability
      Product Discontinued

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IRAK4 [p Thr345] Antibody (A8A8) Summary

Immunogen
A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein.
Modification
p Thr345
Localization
Cytoplasmic
Specificity
This antibody recognizes IRAK-4(pT345) with a phosphorylated site at Threonine 345. It does not crossreact with non-phosphospecific peptide.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IRAK4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1-1 ug/ml
  • Dot Blot 1:100-1:2000
  • ELISA 0.01-0.1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Immunoprecipitation 2-5 ug/ml
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Sterile TBS (pH 7.2) with antibody stabilizer
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRAK4 [p Thr345] Antibody (A8A8)

  • EC 2.7.11
  • EC 2.7.11.1
  • interleukin-1 receptor-associated kinase 4
  • IPD1
  • IRAK4
  • IRAK-4
  • NY-REN-64
  • REN64
  • Renal carcinoma antigen NY-REN-64

Background

IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK familymember necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on IRAK4.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

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Bioinformatics

Gene Symbol IRAK4
Entrez
Uniprot