IRAK1 Antibody


Western Blot: IRAK1 Antibody [NB100-92172] - Western blot (WB) analysis of IRAK1 (T381) antibody in extracts from HeLa cells.

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IRAK1 Antibody Summary

Synthetic peptide, corresponding to amino acids 350-400 of Human IRAK1.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
Western blot (WB) analysis of IRAK1 (T381) pAb in extracts from HeLa cells.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
IRAK1 Lysate (NBL1-12025)

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for IRAK1 Antibody

  • EC 2.7.11
  • EC
  • Il1rak
  • interleukin-1 receptor-associated kinase 1
  • IRAK1
  • IRAK-1
  • IRAKPelle homolog
  • Pelle
  • Plpk


Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1alpha and IL-1beta, and a specific receptor antagonist, IL-1Ralpha. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFkappaB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFkappaB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkappaB.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol IRAK1