Intra Acrosomal Protein Antibody (ACR-4)

Product Discontinued

Intra Acrosomal Protein Antibody (ACR-4) Summary

• Immunogen: Tissue / cell preparation (Pig). Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for imunization.
• Specificity: Intra Acrosomal Protein (ACR-4)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACRV1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Application Notes: Immunocytochemistry, Western Blot: Predicted molecular weight: 32 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Cow and Pig.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Intra Acrosomal Protein Antibody (ACR-4)

• acrosomal protein SP-10
• acrosomal vesicle protein 1D11S4365
• SP-10
• SPACA2
• sperm protein 10

Background

The antibody ACR-4 reacts with bull intra-acrosomal sperm protein, a 25-27 kDa testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACRV1