Interleukin 34 Antibody

Images

 
Western Blot: Interleukin 34 Antibody [NBP1-76356] - Analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western Blot: Interleukin 34 Antibody [NBP1-76356] - Analysis of 125 ng of recombinant human IL-34 with IL-34 antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Interleukin 34 Antibody Summary

Immunogen
Antibody was raised against a 13 amino acid synthetic peptide from near the center of human IL-34. The immunogen is located within amino acids 60 - 110 of IL-34.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL34
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
Application Notes
IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 ug/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Interleukin 34 Peptide (NBP1-76356PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Interleukin 34 Antibody

  • chromosome 16 open reading frame 77
  • IL-34C16orf77MGC34647
  • interleukin 34
  • interleukin-34

Background

Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL34
Uniprot