Recombinant Human Interferon alpha Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

Order Details

Recombinant Human Interferon alpha Protein Summary

Description
Recombinant biologically active Interferon alpha protein.

Source:E. coli

Preparation
Method
Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of IFN-alpha(2a) as a Reference Standard.
Details of Functionality
IFN-alpha 2a is fully biologically active when compared to standard.The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 10^8 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IFNA1
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 ng/ug (IEU/ug) of rHuIFN- 2a.

Applications/Dilutions

Theoretical MW
19.24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-26551 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized without additives.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized 18 M omega-cm water to a final concentration of at least 0.1 mg/ml.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Human Interferon alpha Protein

  • IFN alpha

Background

IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Interferon alpha Recombinant Protein (NBP2-26551)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: In vitro.


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Bioinformatics

Gene Symbol IFNA1