Integrin beta 4/CD104 Antibody (346-11A)

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Flow Cytometry: Integrin beta 4/CD104 Antibody (346-11A) [NB100-65600] - Analysis using the FITC conjugate of NB100-65600. Staining of Mouse thymocytes.
Flow Cytometry: Integrin beta 4/CD104 Antibody (346-11A) [NB100-65600] - Mouse thymocytes.
Flow Cytometry: Integrin beta 4/CD104 Antibody (346-11A) [NB100-65600] - Analysis using the Biotin conjugate of NB100-65600. Staining of Mouse thymocytes.

Product Details

Summary
Product Discontinued
View other related Integrin beta 4/CD104 Primary Antibodies

Order Details


    • Catalog Number
      NB100-65600
    • Availability
      Product Discontinued

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Integrin beta 4/CD104 Antibody (346-11A) Summary

Immunogen
TSP-180 purified from Balb/c murine carcinoma.
Localization
Cell Membrane; single pass type I membrane protein
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ITGB4
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:50 - 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Publications
Read Publication using NB100-65600.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Integrin beta 4/CD104 Antibody (346-11A)

  • CD104 antigen
  • CD104
  • GP150
  • Integrin beta 4
  • integrin beta-4 subunit
  • integrin beta-4
  • integrin, beta 4
  • ITGB4

Background

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities. Integrin beta 4 (CD104), a 200kD glycoprotein that can associate with integrin alpha 6 (CD49f) to form a heterodimer on the cell surface. Expression of CD104 is localised to the basal region of a wide variety of epithelial cells. In mice, CD104 is also expressed on peripheral neurones, carcinoma cell lines and a subset of endothelial cells. The alpha 6 beta 4 (CD49f/CD104) integrin has been identified as a component of hemidesmosomes in stratified squamous epithelium where it plays an important role in the adhesion of epithelia to basement membrane. Studies suggest that the CD49f/CD104 integrin is involved in cell matrix adhesion but expression of the CD49f/CD104 integrin in endothelial cells is thought to have a different function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 4/CD104 Antibody (NB100-65600)(1)


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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65600AF405
Alexa Fluor 488 Labeled NB100-65600AF488
Alexa Fluor 647 Labeled NB100-65600AF647
Alexa Fluor 700 Labeled NB100-65600AF700
Allophycocyanin Labeled NB100-65600APC
DyLight 350 Labeled NB100-65600UV
DyLight 405 Labeled NB100-65600V
DyLight 488 Labeled NB100-65600G
DyLight 680 Labeled NB100-65600FR
DyLight 755 Labeled NB100-65600IR
PE Labeled NB100-65600PE
PerCP Labeled NB100-65600PCP

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Bioinformatics

Gene Symbol ITGB4
Entrez
Uniprot