Integrin beta 3/CD61 Antibody (256809) [Unconjugated]

PBMC monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Integrin beta 3/CD61 Monoclonal Antibody (Catalog # MAB2266) or (B) isotype control antibody (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 256809
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 3/CD61 Antibody (256809) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 3/CD61
  Gly27-Asp718
  Accession # P05106
• Specificity: Detects human Integrin beta 3/CD61 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin beta 1, beta 2, recombinant mouse Integrin beta 4, or beta 6 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 3/CD61 Antibody (256809) [Unconjugated]

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

Integrin beta 3, also called CD61, associates with the Integrin alpha IIb or alpha V subunits to form heterodimeric receptors for collagens, fibronectin, vitronectin, fibrinogen, Von willebrand factor or thrombospondin. alpha IIb/ beta 3, also known as GPIIb IIIa (Glycoprotein IIb-IIIa), is expressed on platelets. alpha V/ beta 3, also known as vitronectin receptor, is expressed on endothelial cells, some B cells, platelets and monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 3/CD61 Antibody (MAB2266)(3)

Publications using MAB2266

• H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma BMC gastroenterology, 2022-03-28;22(1):153. 2022-03-28 [PMID: 35350978] (ELISA Detection, Human)
• H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays FEBS Open Bio, 2021-02-03;0(0):. 2021-02-03 [PMID: 33345458] (ELISA Detection, Human)
• Burton VJ, Butler LM, McGettrick HM, Stone PC, Jeffery HC, Savage CO, Rainger GE, Nash GB Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture. Exp. Cell Res., 2010-11-04;317(3):276-92. 2010-11-04 [PMID: 21056557] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGB3
• Uniprot:
  • P05106()