Integrin beta 1/CD29 Antibody (HMb1-1) [PE]

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Flow Cytometry: Integrin beta 1/CD29 Antibody (HMb1-1) [PE] [NBP1-43874] - See Notes

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
HMb1-1
Clonality
Monoclonal
Host
Armenian Hamster
Concentration
0.2 mg/ml

Order Details

Integrin beta 1/CD29 Antibody (HMb1-1) [PE] Summary

Immunogen
The immunogen for this antibody was CD29.
Marker
Stem Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
ITGB1
Purity
Protein A or G purified
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
PE
Format
PE (A=488, E=575)
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This HMb1-1 antibody has been tested by flow cytometric analysis of mouse spleen, thymus and bone marrow cell suspensions. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Alternate Names for Integrin beta 1/CD29 Antibody (HMb1-1) [PE]

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1/CD29
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

The HMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alphabeta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Integrin beta 1/CD29 Antibody (NBP1-43874). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies

 

Isotype Controls

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol ITGB1
Entrez

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