Integrin beta 1/CD29 Antibody (DF5)


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Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
0.1 mg/ml

Order Details

Integrin beta 1/CD29 Antibody (DF5) Summary

Monoclonal antibody (Mab) to b1 integrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Wheat germ agglutinin binding glycoproteins of human fibroblasts were used as immunogen.
Stem Cell Marker
The antibody reacts with the b1 subunit of the integrin protein family and seems to be human specific. The antibody reacts with an extracellular epitope of the b1 integrin molecule.
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS solution containing 1.0% w/v BSA
0.1% Sodium Azide
0.1 mg/ml
Protein A or G purified


  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemical stainings on frozen and paraffin sections, western blotting and other immunoassays for cellular and tumor biology.
Read Publication using NBP1-42319.

Reactivity Notes


Alternate Names for Integrin beta 1/CD29 Antibody (DF5)

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta


Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4). beta1 -Integrins mediate cell-extracellular matrix interactions and share a common b-subunit. Monoclonal antibodies against this beta1 -Integrin subunit are applicable in paraffin-embedded and frozen sections, immunoblotting and immunoprecipitation. Numerous studies have shown that beta1 -Integrins have a distinct and wide cellular distribution and that there are alterations in malignant cells in their beta1-integrin expression.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 1/CD29 Antibody (NBP1-42319)(1)

We have publications tested in 1 application: IHC-P.

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Publication using NBP1-42319 Applications Species
Bernardini N et al. Tumori 83, 673-678. 1997 [PMID:9267487] (IHC-P) IHC-P

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NBP1-42319). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies


Isotype Controls

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

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Product PDFs


Gene Symbol ITGB1

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