Integrin beta 1/CD29 Antibody (4B7R)

Images

 
Flow Cytometry: Integrin beta 1/CD29 Antibody (4B7R) [NB100-65803] - Analysis using the Biotin conjugate of NB100-65803. Staining of human peripheral blood lymphocytes.
Flow Cytometry: Integrin beta 1/CD29 Antibody (4B7R) [NB100-65803] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD29.
Flow Cytometry: Integrin beta 1/CD29 Antibody (4B7R) [NB100-65803] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD29: RPE.
Flow Cytometry: Integrin beta 1/CD29 Antibody (4B7R) [NB100-65803] - Analysis using the FITC conjugate of NB100-65803. Staining of human peripheral blood lymphocytes.

Product Details

Summary
Product Discontinued
View other related Integrin beta 1/CD29 Primary Antibodies

Order Details


    • Catalog Number
      NB100-65803
    • Availability
      Product Discontinued

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Integrin beta 1/CD29 Antibody (4B7R) Summary

Immunogen
Occular melanoma cell line V+B2
Localization
Type 1 membrane protein
Marker
Stem Cell Marker
Specificity
NB100-65803 recognises the beta 1 subunit of human integrin (CD29), which is a transmembrane glycoprotein expressed by most leucocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Positive Control
Integrin beta 1/CD29 Lysate (NBL1-12069)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Integrin beta 1/CD29 Antibody (4B7R)

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Integrin beta 1/CD29 Antibody (NB100-65803). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Integrin beta 1/CD29 Products

Bioinformatics Tool for Integrin beta 1/CD29 Antibody (NB100-65803)

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1
Entrez