Integrin alpha 5/CD49e Antibody (NKI-SAM-1) [Biotin]

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Flow Cytometry: Integrin alpha 5/CD49e Antibody (NKI-SAM-1) [Biotin] [NB100-78109] - Human peripheral blood lymphocytes stained with biotinylated NKI-SAM-1, followed by Sav-PE

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78109
    • Availability
      Product Discontinued

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Integrin alpha 5/CD49e Antibody (NKI-SAM-1) [Biotin] Summary

Immunogen
U937 cells
Specificity
CD49e (NKI-SAM-1)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ITGA5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.
Readout System
Positive Control
Integrin alpha 5/CD49e Lysate (NBL1-12065)

Reactivity Notes

Cynomolgus Macaque.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin alpha 5/CD49e Antibody (NKI-SAM-1) [Biotin]

  • CD49 antigen-like family member E
  • CD49e antigen
  • CD49e
  • Fibronectin receptor subunit alpha
  • fibronectin receptor, alpha subunit
  • FNRAVLA5A
  • Integrin alpha 5
  • integrin alpha-5
  • Integrin alpha-F
  • integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
  • ITGA5
  • very late activation protein 5, alpha subunit
  • VLA-5 alpha
  • VLA-5

Background

Integrin alpha 5 is a type I integral membrane glycoprotein, known as Integrin alpha 5, VLA-5 alpha chain. It forms noncovalent heterodimer with integrin beta1 (CD29). CD49e contains two disulfide-linked subunits of 135 kDa and 24 kDa, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. Integrin alpha 5/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Integrin alpha 5/CD49e Products

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Bioinformatics

Gene Symbol ITGA5
Entrez