Integrin alpha 4/CD49d Antibody (MRalpha4-1)

Product Discontinued

Integrin alpha 4/CD49d Antibody (MRalpha4-1) Summary

• Immunogen: RBL-2H3 rat basophilic leukaemia cell line.
• Specificity: NBP1-28812 specifically recognises rat CD49d, otherwise known as integrin alpha 4 chain/VLA-4, a 150kDa type-1 transmembrane glycoprotein and member of the integrin alpha chain family, which acts as a receptor for fibronectin and CD106 (VCAM-1).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:100
  • Functional
  • Immunoprecipitation
• Application Notes: Clone MRalpha4-1 inhibits the in vitro and in vivo functions of CD49d. Studies have shown that in rat Peyer's patches, clone MRalpha4-1 inhibits the rolling and adherence of lymphocytes to postcapillary venules (PCV), and significantly inhibits the transendothelial migration of T lymphocytes. For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

Reactivity Notes

Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Notes

This is a low endotoxin product. Endotoxin level <0.01EU/ug

Alternate Names for Integrin alpha 4/CD49d Antibody (MRalpha4-1)

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

This antibody specifically recognises rat CD49d, otherwise known as integrin alpha 4 chain/VLA-4, a 150kDa type-1 transmembrane glycoprotein and member of the integrin alpha chain family, which acts as a receptor for fibronectin and CD106 (VCAM-1). CD49d is expressed broadly on T and B lymphocytes, monocytes, thymocytes, mast cells and dendritic cells and is involved in cell-cell interactions, mononuclear cell trafficking to sites of endothelial inflammation, and the adhesion of cells to the extracellular matrix. Clone MRalpha4-1 inhibits the in vitro and in vivo functions of CD49d. Studies have shown that in rat Peyer's patches, clone MRalpha4-1 inhibits the rolling and adherence of lymphocytes to postcapillary venules (PCV), and significantly inhibits the transendothelial migration of T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 4/CD49d Antibody (NBP1-28812)(2)

Publications using NBP1-28812

• Tsuzuki, Y et al. Alpha4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer’s patches of rats. Int Immunol 8: 287-295. [PMID: 8671614]
• Yasuda, M et al. Expression and function of fibronectin binding integrins on rat mast cells. Int Immunol 7: 251-258. [PMID: 7734420]

Bioinformatics

• Gene Symbol: ITGA4