Integrin alpha 1/CD49a Antibody (639508) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 639508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Integrin alpha 1/CD49a Antibody (639508) [Alexa Fluor® 594] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived human Integrin α1/CD49a
  Phe29-Pro1141
  Accession # P56199
• Specificity: Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha 2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha 6x1/ beta 4, alpha D/ beta 2, alpha 7x2/ beta 1, alpha E/ beta 7, or alpha 2b/ beta 3 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 1/CD49a Antibody (639508) [Alexa Fluor® 594]

• CD49 antigen-like family member A
• CD49a antigen
• CD49a
• Integrin alpha 1
• integrin, alpha 1
• ITGA1
• Laminin and collagen receptor
• very late activation protein 1
• VLA-1
• VLA1integrin alpha-1

Background

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules.  It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.  Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.  It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.  Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail.  The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites.  Potential splice variants exist that show a two and four aa substitution for aa 765-1179.  Over aa 29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.