iNOS Antibody

Images

 
Western Blot: iNOS Antibody [NBP2-30015] - 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band ...read more
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NBP2-30015] - Cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight (R) 554 Phalloidin at 1/100 dilution (red). The nuclear counter ...read more
Immunohistochemistry-Paraffin: iNOS Antibody [NBP2-30015] - Human brain tissue sections
Flow Cytometry: iNOS Antibody [NBP2-30015] - Analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: iNOS Antibody [NBP2-30015] - 1:2000 dilution + Y79 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band ...read more
Immunocytochemistry/ Immunofluorescence: iNOS Antibody [NBP2-30015] - Hela cells.
Immunohistochemistry-Paraffin: iNOS Antibody [NBP2-30015] - Analysis in human hepatocarcinoma.

Product Details

Summary
Product Discontinued
View other related iNOS Primary Antibodies

Order Details


    • Catalog Number
      NBP2-30015
    • Availability
      Product Discontinued

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iNOS Antibody Summary

Immunogen
This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:25
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry-Paraffin 1:50 - 1:100
Theoretical MW
131.117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
iNOS Lysate (NBP2-65101)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.64 mg/ml
Purity
Protein A purified

Alternate Names for iNOS Antibody

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for iNOS Antibody (NBP2-30015). (Showing 1 - 1 of 1 FAQ).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for iNOS Antibody (NBP2-30015)

Discover related pathways, diseases and genes to iNOS Antibody (NBP2-30015). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for iNOS Antibody (NBP2-30015)

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Pathways for iNOS Antibody (NBP2-30015)

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PTMs for iNOS Antibody (NBP2-30015)

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Research Areas for iNOS Antibody (NBP2-30015)

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Blogs on iNOS.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot