Recombinant E. coli Inorganic Pyrophosphatase/PPA1 Protein

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Product Details

Summary
Reactivity EcSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant E. coli Inorganic Pyrophosphatase/PPA1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-176 of E.coli Inorganic Pyrophosphatase/PPA1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PPA1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant E. coli Inorganic Pyrophosphatase/PPA1 Protein

  • diphosphate phosphohydrolase
  • EC 3.6.1.1
  • inorganic diphosphatase
  • inorganic pyrophosphatase 1
  • inorganic pyrophosphatase
  • IOPPP
  • IOPPPMGC111556
  • IPP1
  • PP1
  • PPA1
  • PPase
  • PPcytosolic inorganic pyrophosphatase
  • pyrophosphatase (inorganic) 1
  • pyrophosphatase (inorganic)
  • pyrophosphatase 1
  • Pyrophosphate phospho-hydrolase
  • SID6-8061

Background

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PPA1
Entrez