InnovaCoat GOLD 40nm Mini Antibody Labeling Kit

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InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0005]
Lateral Flow Assay: InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0005] - A comparison between the signal intensity, measured on a commercially available lateral flow strip reader, for anti-IgA antibodies ...read more
Lateral Flow Assay: InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0005] - A comparison between the percentage binding of anti-IgA antibodies conjugated to 40nm gold particles by a traditional passive method ...read more
Lateral Flow Assay: InnovaCoat GOLD 40nm Mini Antibody Labeling Kit [230-0005] - A comparison between the percentage binding of anti-cortisol antibodies conjugated to 40nm gold particles by a traditional passive method ...read more

Product Details

Summary
Kit Type
Antibody Labeling Kit

Order Details

InnovaCoat GOLD 40nm Mini Antibody Labeling Kit Summary

Description
InnovaCoat GOLD antibody labeling kits enable the covalent attachment of antibodies, proteins, peptides and other biomolecules to gold nanoparticles with a proprietary InnovaCoat coating. The hands-on time is around 30 seconds and the gold conjugate is ready to use after 20 minutes.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
Different nanoparticle sizes, concentrations and chemistries availableFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalableEasy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Proprietary surface coatingCovalent bond, stable conjugates formed
Covalent conjugation uses significantly less antibody than passive conjugationSave precious antibody
Uniform spherical shape and narrow size distributionBatch-to-batch consistency
Kit Type
Antibody Labeling Kit

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1 vial or bottle of reaction buffer
  2. 1 vial or bottle of antibody diluent
  3. 1 vial of quencher
  4. 1 glass vial (Midi kit) OR 3 or 10 cryotubes (Mini kit) of InnovaCoat GOLD (dependent on kit)

Notes

InnovaCoat GOLD® is a registered trademark of Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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FAQs for InnovaCoat GOLD 40nm Mini Kit (230-0005). (Showing 1 - 7 of 7 FAQ).

  1. Can antibodies from different species be used?
    • Yes, the InnovaCoat® system has been tested with antibodies from a variety of species including mouse, rabbit, goat and sheep.
  2. Can antibody fragments be conjugated?
    • Yes. One of the advantages of the protective coat is that it is less likely than a bare metal surface to cause denaturation and loss of affinity of the antibody fragments.
  3. Does the antibody bind to the metal surface?
    • No. The protective surface coat completely shields the metal surface and prevents direct metal-antibody interactions. For this reason, you cannot use InnovaCoat® GOLD for passive conjugation of antibodies.
  4. Is the kit suitable for conjugating analytes and other small molecules?
    • Yes, but please contact Innova technical services. Depending on the functional groups on your analyte you may need a different type of surface or different chemistry for optimal results.
  5. What can I do if my antibody preparation contains interfering substances
    • The simplest procedure is to dialyse the antibody against a suitable buffer. Relatively weak buffers (e.g. 20mM) are preferred so that the pH conditions of the covalent reaction are not significantly altered upon addition of the antibody. See also the comments in Section 3 of the protocol. If dialysis is not practical you can use other popular methods of buffer exchange if required e.g. desalting columns.
  6. What if I need bulk material?
    • The kit that you have purchased is a convenience product for rapid production of small quantities of conjugate for screening purposes. Bulk quantities of InnovaCoat® GOLD can be provided in a variety of formats. Please enquire.
  7. What type of linkage to gold is formed?
    • The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat® surface.

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InnovaCoat GOLD 40nm Mini 230-0005

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