ING1 Antibody - BSA Free

Western Blot: ING1 Antibody [NB600-908] - Western blot analysis is shown using Affinity Purified ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1, ~33kDa) and ING1c protein (lane 2, ~24kDa). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques.

N/A: ING1 Antibody [NB600-908]

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

ING1 Antibody - BSA Free Summary

• Description: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified ING1 Antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human ING1 protein (Inhibitor of growth family, member 1). (Uniprot: Q9UK53)
• Localization: Nuclear
• Specificity: Reactivity occurs against human p33 ING11 protein and is 100% homologous to all splice variants for ING11. Cross reactivity with p33 ING11 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING11 proteins from other sources is not known.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ING1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000-1:14000
  • Western Blot 1:500-1:3000
• Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blotting using over expressed cell lysates. Expect a band approximately 47 kDa, 33 kDa or 24 kDa in size corresponding to variants of ING1 by western blotting in the appropriate cell lysate or extract. Specific conditions for other applications should be optimized by the end user.
• Control Peptide:

  ING1 Protein (NBP1-96943)

Reactivity Notes

Reactivity occurs against human p33 ING11 protein and is 100% homologous to all splice variants for ING11. Cross reactivity with p33 ING11 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING11 proteins from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ING1 Antibody - BSA Free

• growth inhibitor ING1
• growth inhibitory protein ING1
• ING1
• inhibitor of growth family, member 1
• inhibitor of growth protein 1
• p24ING1c
• p33
• p33ING1
• p33ING1b
• p47
• p47ING1a
• tumor suppressor ING1

Background

p33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodes the longest isoform (D). Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ING1
• Entrez:
  • 3621(Human)
• Uniprot:
  • Q9UK53(Human)