Recombinant Influenza A Virus H5N1 Influenza A H5N1 His Protein

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SDS-Page: Recombinant Virus Influenza A Haemagglutinin H5 Protein [NBP1-99028] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity VSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Influenza A Virus H5N1 Influenza A H5N1 His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 17-338 of Influenza A (H5N1) Influenza A Haemagglutinin H5

Source: E.coli

Amino Acid Sequence: ADPMDQICIG YHANNSTEQV DTIMEKNVTV THAQDILEKT HNGKLCDLDG VKPLILRDCS VAGWLLGNPM CDEFINVPEW SYIVEKANPA NDLCYPGNFN DYEELKHLLS RINHFEKIQI IPKSSWSDHE ASSGVSSACP YQGVPSFFRN VVWLIKKNNT YPTIKRSYNN TNQEDLLILW GIHHSNDAAE QTKLYQNPTT YISVGTSTLN QRLVPKIATR SKVNGQSGRM DFFWTILKPN DAINFESNGN FIAPEYAYKI VKKGDSAIMK SEVEYGNCNT KCQTPIGAIN SSMPFHNIHP LTIGECPKYV KSNKLVLATG LRNSPLHHHH HH

Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
HA
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Influenza A virus

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Influenza A Virus H5N1 Influenza A H5N1 His Protein

  • HA
  • Hemagglutinin

Background

H5N1 is a subtype of the species Influenza A virus of the Influenzavirus A genus of the Orthomyxoviridae family. consists of single-stranded eight-segment negative-sense genomic RNAs, helical viral ribonucleoprotein (RNP) complexes (RNA segments NP, PB2, PB1 and PA), three viral envelope proteins (hemagglutinin [HA], neuraminidase [NA], and M2 ion channel), and a maxtir (M1) protein. Influenza A viruses are further classified into 16 HA (H1-H16) and 9 NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelope glycoproteins. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Influenza A H5N1 Recombinant Protein (NBP1-99028) (0)

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FAQs for Influenza A H5N1 Recombinant Protein (NBP1-99028). (Showing 1 - 1 of 1 FAQ).

  1.  I would like to know this Hemaglutinin is produced in E coli. Is the protein without glicosilation. glicosilation is important immunogen, so what is the purpose to produce the protein without glicosilation,Is it also usfull as antigen?
    • Yest, NBP1-99028 is a recombinant protein produced by E.coli. Therefore, we expect it to be without glycoslyation. Depending on the protein, glycosylation may be important. Some proteins do not require glycoslyation for it to be folded properly and/or be fully functional. E.coli production is more economical compared to using mammlian cell lines. In this case, NBP1-99028 is designed to be used for PAGE applications. As the protein is mainly used in a state that is denatured, it is not required to be structurally intact.

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Bioinformatics

Gene Symbol HA