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Indoleamine 2,3-dioxygenase/IDO Antibody

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Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Molt-4 D: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Molt-4 D: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-33590
    • Availability
      Product Discontinued

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Indoleamine 2,3-dioxygenase/IDO Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IDO1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDO1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody

  • 3dioxygenase
  • EC 1.13.11.52
  • IDO
  • IDO1
  • IDOIDO-1
  • INDO
  • INDOindole 2,3-dioxygenase
  • Indoleamine 2
  • indoleamine 2,3-dioxygenase 1
  • Indoleamine 2,3-dioxygenase
  • indoleamine-pyrrole 2,3 dioxygenase
  • Indoleamine-pyrrole 2,3-dioxygenase

Background

T lymphocytes undergo proliferation arrest when exposed to tryptophan shortage, which can be provoked by indoleamine 2,3-dioxygenase (IDO), an enzyme that is expressed in placenta and catalyzes tryptophan degradation. Here we show that most human tumors constitutively express IDO. Results suggest that the efficacy of therapeutic vaccination of cancer patients might be improved by concomitant administration of an IDO inhibitor (1). Certain macrophages, induced to express IDO in response to interferon-gamma and other signals from activating T cells, inhibit T-cell proliferation in vitro by rapidly consuming tryptophan. Because IDO is also expressed by human syncytiotrophoblast cells and systemic tryptophan concentration falls during normal pregnancy, it is believed that IDO expression at the maternal-fetal interface is necessary to prevent immunologic rejection of the fetal allografts (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.
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Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Western blot of crude rat brain extract from a postnatal 3 day animal stained with NBP1-92684. Two bands at ~45kDa and ~35kDa show that NBP1-92684 binds to an epitope in the region of Doublecortin shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.
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Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.
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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Western Blot: DLX3 Antibody [NBP2-24646] - Analysis of DLX3 in NIH 3T3 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24646 at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
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Indoleamine
2,3-dioxygenase/IDO A ...
NBP1-33590
Indoleamine 2,3-dioxygenase/IDO Antibody
Species: Hu
Applications: WB, ICC/IF

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Bioinformatics

Gene Symbol IDO1
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