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Indoleamine 2,3-dioxygenase/IDO Antibody

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Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with IDO1 antibody diluted at 1:3000 diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with IDO1 antibody ...read more

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-33590
    • Availability
      Product Discontinued

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Indoleamine 2,3-dioxygenase/IDO Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IDO1. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDO1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody

  • 3dioxygenase
  • EC 1.13.11.52
  • IDO
  • IDO1
  • IDOIDO-1
  • INDO
  • INDOindole 2,3-dioxygenase
  • Indoleamine 2
  • indoleamine 2,3-dioxygenase 1
  • Indoleamine 2,3-dioxygenase
  • indoleamine-pyrrole 2,3 dioxygenase
  • Indoleamine-pyrrole 2,3-dioxygenase

Background

T lymphocytes undergo proliferation arrest when exposed to tryptophan shortage, which can be provoked by indoleamine 2,3-dioxygenase (IDO), an enzyme that is expressed in placenta and catalyzes tryptophan degradation. Here we show that most human tumors constitutively express IDO. Results suggest that the efficacy of therapeutic vaccination of cancer patients might be improved by concomitant administration of an IDO inhibitor (1). Certain macrophages, induced to express IDO in response to interferon-gamma and other signals from activating T cells, inhibit T-cell proliferation in vitro by rapidly consuming tryptophan. Because IDO is also expressed by human syncytiotrophoblast cells and systemic tryptophan concentration falls during normal pregnancy, it is believed that IDO expression at the maternal-fetal interface is necessary to prevent immunologic rejection of the fetal allografts (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.
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Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.
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Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.
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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
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Indoleamine
2,3-dioxygenase/IDO A ...
NBP1-33590
Indoleamine 2,3-dioxygenase/IDO Antibody
Species: Hu, RM
Applications: WB

Publications for Indoleamine 2,3-dioxygenase/IDO Antibody (NBP1-33590) (0)

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Bioinformatics

Gene Symbol IDO1
Entrez