Indoleamine 2,3-dioxygenase/IDO Antibody

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Molt-4 D: Raji 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-33590] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Product Discontinued

Indoleamine 2,3-dioxygenase/IDO Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IDO1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IDO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody

• 3dioxygenase
• EC 1.13.11.52
• IDO1
• IDOIDO-1
• INDO
• INDOindole 2,3-dioxygenase
• Indoleamine 2
• indoleamine 2,3-dioxygenase 1
• Indoleamine 2,3-dioxygenase
• indoleamine-pyrrole 2,3 dioxygenase
• Indoleamine-pyrrole 2,3-dioxygenase

Background

T lymphocytes undergo proliferation arrest when exposed to tryptophan shortage, which can be provoked by indoleamine 2,3-dioxygenase (IDO), an enzyme that is expressed in placenta and catalyzes tryptophan degradation. Here we show that most human tumors constitutively express IDO. Results suggest that the efficacy of therapeutic vaccination of cancer patients might be improved by concomitant administration of an IDO inhibitor (1). Certain macrophages, induced to express IDO in response to interferon-gamma and other signals from activating T cells, inhibit T-cell proliferation in vitro by rapidly consuming tryptophan. Because IDO is also expressed by human syncytiotrophoblast cells and systemic tryptophan concentration falls during normal pregnancy, it is believed that IDO expression at the maternal-fetal interface is necessary to prevent immunologic rejection of the fetal allografts (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDO1
• Entrez:
  • 3620(Human)
  • 15930(Mouse)