Importin beta/KPNB1 Antibody

Images

 
Western Blot: Importin beta/KPNB1 Antibody [NB100-40784] - Staining of A431 (A), HEK293 (B), Jurkat (C) cell lysates with antibody at 0.03 ug/mL, Daudi (D), HeLa (E) cell lysates with antibody at 0.01 ug/mL, and Kelly ...read more
Immunocytochemistry/ Immunofluorescence: Importin beta/KPNB1 Antibody [NB100-40784] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more
Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NB100-40784] - Staining of paraffin embedded Human Spleen with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Importin beta/KPNB1 Antibody [NB100-40784] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Importin beta/KPNB1 Antibody Summary

Immunogen
Peptide with sequence C-RRSKTNKAKTLAT corresponding to C-Terminus according to NP_002256.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KPNB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.1 ug/mL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
Publications
Read Publication using NB100-40784.

Reactivity Notes

Expected from sequence similarity: Mouse, Canine, Bovine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin beta/KPNB1 Antibody

  • IMB1
  • Impnb
  • Importin 1
  • importin 90
  • Importin beta
  • importin beta-1 subunit
  • importin subunit beta-1
  • importin-90
  • IPO1
  • IPOB
  • karyopherin (importin) beta 1
  • Karyopherin subunit beta-1
  • KPNB1
  • MGC2155
  • MGC2156
  • MGC2157
  • NTF97
  • NTF97Pore targeting complex 97 kDa subunit
  • Nuclear factor p97
  • PTAC97

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KPNB1
Entrez
Uniprot