Imm-Link Ovalbumin (Amine) Immunogen labeling Kit Summary
BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.
The imm-Link amine conjugation kit allows amine containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.
|Immunogen Kit ||Amine |
|Target Functional Groups ||Lysines or free amines NH2 |
- Easy to use
- All components supplied
- Wide range of chemistries
- Hands on time to set up conjugation reaction is 20-30 seconds
- Supplied dialysis cartridge ensures conjugates are recovered in high yield
- Full technical support available
Immunogen labeling Kit
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
- 1 or 3 glass vial(s) of Imm-Link mix
- 1 or 3 250 ml bottle(s) of 10x dialysis buffer
- 1 or 3 dialysis cartridge(s)
- 1 or 3 vial(s) of Imm-Link Amine Modifier reagent
Imm-Link® is a registered trademark of Innova Biosciences.
The Imm-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make carrier protein conjugates with minimal hands-on time - less than 30 seconds. imm-Link simplifies amine conjugation allowing amine-containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing both the carrier protein and all of the chemicals required to form the conjugate. Upon dissolution of the Imm-Link mixture, proprietary chemicals in the mixture become activated, resulting in the coupling of the hapten to the carrier protein in a gentle and controlled process. Despite its simplicity, imm-Link is a very sophisticated conjugation system. Once the conjugation reaction is complete, the hapten- carrier conjugate is dialysed using the supplied dialysis cartridge to remove unwanted by-products with ease from the conjugation reaction. The hapten conjugate can then be used for the purpose of antibody production. The design of the dialysis cartridge ensures that the hapten conjugate is recovered in high yield.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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