Imm-Link KLH (Sulfhydryl) Immunogen labeling Kit

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Product Details

Summary

Order Details

Imm-Link KLH (Sulfhydryl) Immunogen labeling Kit Summary

Description
BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.

The imm-Link sulfhydryl conjugation kit allows sulfhydryl containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.

Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.

Immunogen Kit Sulfhydryl
Target Functional Groups Cysteines or free sulfhydryl groups (SH)


Features:
  • Easy to use
  • All components supplied
  • Wide range of chemistries
  • Hands on time to set up conjugation reaction is 20-30 seconds
  • Supplied dialysis cartridge ensures conjugates are recovered in high yield
  • Full technical support available
Kit Type
Immunogen labeling Kit

Applications/Dilutions

Application Notes
Target Functional Groups: Cysteines or free sulfhydryl groups (SH).
Reviewed Applications
Read 1 Review rated 5
using
460-0500 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 1 or 3 glass vial(s) of Imm-Link mix
  2. 1 or 3 dialysis cartridge(s)
  3. 1 or 3 250 ml bottle(s) of 10x dialysis buffer
  4. 1 or 3 vial(s) of Imm-Link Sulfhydryl Modifier reagent

Notes

Imm-Link® is a registered trademark of Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Review for Imm-Link KLH (Sulfhydryl) Kit (460-0500) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ELISA.

Reviews using 460-0500:
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reviewed by:
Robert Hnasko
ELISA Mouse 04/18/2011
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Summary

ApplicationELISA
Sample TestedPeptide
SpeciesMouse
CommentsWorked as described for peptide conjugation at Cysteine used in immunization - fast and cost effective post synthesis

Comments

CommentsWorked as described for peptide conjugation at Cysteine used in immunization - fast and cost effective post synthesis

Product General Protocols

View specific protocols for Imm-Link KLH (Sulfhydryl) Kit (460-0500): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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Additional Imm-Link KLH (Sulfhydryl) Products

Imm-Link KLH (Sulfhydryl) 460-0500

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Recent Reviews

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Robert Hnasko
04/18/2011
Application: ELISA
Species: Mouse