ILF2 Antibody (6F1)

Images

 
Western Blot: ILF2 Antibody (6F1) [NBP1-47811] Analysis of extracts (35ug) from 9 different cell lines by using anti-ILF2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ILF2 Antibody (6F1) [NBP1-47811] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ILF2.
Immunohistochemistry-Paraffin: ILF2 Antibody (6F1) [NBP1-47811] - Staining of paraffin-embedded Kidney tissue using anti-ILF2 mouse monoclonal antibody.
Western Blot: ILF2 Antibody (6F1) [NBP1-47811] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
6F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ILF2 Antibody (6F1) Summary

Immunogen
Protein expressed in 293T cell transfected with human ILF2 expression vector
Specificity
This antibody is specific for Homo sapiens interleukin enhancer binding factor 2, 45kDa (ILF2).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ILF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Canine

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ILF2 Antibody (6F1)

  • interleukin enhancer binding factor 2, 45kD
  • interleukin enhancer binding factor 2, 45kDa
  • interleukin enhancer-binding factor 2
  • MGC8391
  • NF45PRO3063
  • Nuclear factor of activated T-cells 45 kDa
  • nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2.In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of thisgene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ILF2
Entrez