IL-7 R alpha/CD127 [p Tyr449] Antibody

Images

 
Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. ...read more
Western Blot: IL-7 R alpha/CD127 [p Tyr449] Antibody [NB110-85537] - Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4C. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

IL-7 R alpha/CD127 [p Tyr449] Antibody Summary

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Modification
p Tyr449
Specificity
This is specific for mouse IL-7 protein phosphorylated at Y449. A BLAST analysis was used to suggest cross-reactivity with IL-7 from human and rat sources based on 100% identity with the immunizing sequence.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL7R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • ELISA 1:5000-1:50000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation
Application Notes
Expect a band approximately 55 kDa in size corresponding - phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Immunopreciptiation should be user optimized for best results.

Reactivity Notes

Reacts with Human, Mouse, and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IL-7 R alpha/CD127 [p Tyr449] Antibody

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-39002
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
AF1086
Species: Hu
Applications: WB, Flow, IHC, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-42795
Species: Hu
Applications: WB, Flow, Func, IHC, IHC-Fr, IP, CyTOF-ready
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
AF284
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
NB110-85537
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IP

Publications for IL-7 R alpha/CD127 Antibody (NB110-85537) (0)

There are no publications for IL-7 R alpha/CD127 Antibody (NB110-85537).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-7 R alpha/CD127 Antibody (NB110-85537) (0)

There are no reviews for IL-7 R alpha/CD127 Antibody (NB110-85537). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-7 R alpha/CD127 Antibody (NB110-85537) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IL-7 R alpha/CD127 Products

Bioinformatics Tool for IL-7 R alpha/CD127 Antibody (NB110-85537)

Discover related pathways, diseases and genes to IL-7 R alpha/CD127 Antibody (NB110-85537). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-7 R alpha/CD127 Antibody (NB110-85537)

Discover more about diseases related to IL-7 R alpha/CD127 Antibody (NB110-85537).
 

Pathways for IL-7 R alpha/CD127 Antibody (NB110-85537)

View related products by pathway.

PTMs for IL-7 R alpha/CD127 Antibody (NB110-85537)

Learn more about PTMs related to IL-7 R alpha/CD127 Antibody (NB110-85537).
 

Research Areas for IL-7 R alpha/CD127 Antibody (NB110-85537)

Find related products by research area.

Blogs on IL-7 R alpha/CD127

There are no specific blogs for IL-7 R alpha/CD127, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-7 R alpha/CD127 [p Tyr449] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol IL7R
Entrez