IL-7 R alpha/CD127 Antibody (22F3D9) [PE]

Images

 
Flow Cytometry: IL-7 R alpha/CD127 Antibody (22F3D9) [PE] [NBP2-22376PE] - analysis of IL7 Receptor alpha in human PBMC using anti-IL7 Receptor alpha antibody. The primary antibody was used at a dilution of 1:100, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
22F3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

IL-7 R alpha/CD127 Antibody (22F3D9) [PE] Summary

Immunogen
Amino acids 141-237 of human CD127 were used as the immunogen for the antibody.
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
IL7R
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.The amino acid sequence used as immunogen for the antibody is present in two isoforms of CD127: 459 amino acids and 299 amino acids.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22376PE in the following application:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IL-7 R alpha/CD127 Antibody (22F3D9) [PE]

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-7 R alpha/CD127 Antibody (NBP2-22376PE) (0)

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Review for IL-7 R alpha/CD127 Antibody (NBP2-22376PE) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-22376PE:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry IL-7 R alpha/CD127 NBP2-22376PE
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reviewed by:
Anonymous
Flow Human 11/18/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

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FAQs for IL-7 R alpha/CD127 Antibody (NBP2-22376PE) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-22376APC
Biotin Labeled NBP2-22376B
DyLight 350 Labeled NBP2-22376UV
DyLight 405 Labeled NBP2-22376V
DyLight 488 Labeled NBP2-22376G
DyLight 550 Labeled NBP2-22376R
DyLight 650 Labeled NBP2-22376C
DyLight 680 Labeled NBP2-22376FR
DyLight 755 Labeled NBP2-22376IR
HRP Labeled NBP2-22376H

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Recent Reviews

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1
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Anonymous
11/18/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IL7R
Entrez