Recombinant Human IL-6 Protein

Product Discontinued

Recombinant Human IL-6 Protein Summary

• Description: A DNA sequence encoding the human Interleukin 6 protein containing the signal peptide and the mature human Interleukin 6.
  
  

  
  
  Source: Modified human 293 cells

• Specificity: IL-6 HCX migrates as a broad band between 20 and 25 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-6 that has a predicted molecular mass of 21.0kDa. Purified IL-6 HCX consists of 0-20% carbohydrate by weight.
• Preparation Method: A DNA sequence encoding the human Interleukin 6 protein sequence (containing the signal peptide sequence, and the mature human Interleukin 6 sequence) was expressed in modified human 293 cells.
• Details of Functionality: The ED50 of IL-6 HCX is typically 0.15 - 0.35 ng/ml as measured in a cell proliferation assay using a human growth factor-dependent TF-1 cell line.
• Protein/Peptide Type: Biologically Active Protein
• Gene: IL6

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Functional
  • Western Blot
• Application Notes: IL-6 HCX separates into a number of isoforms with a pI between 5.5 and 7.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-6 that has a predicted pI of 6.22.

Reactivity Notes

This is a Human protein

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1% human serum albumin and 10% trehalose.
• Concentration: 0.02 mg/ml

Notes

Disclaimer Note -Disclaimer Note - Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits. Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Alternate Names for Recombinant Human IL-6 Protein

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

IL-6 is a pleotropic cytokine that regulates the development, proliferation and maturation of a number of hematopoietic cells and is essential for the maturation of B cells into immunoglobulin-secreting cells. IL-6 can also influence the growth and function of non-hematopoietic cells including the differentiation of nerve cells, metabolism of bone and induction of acute phase proteins in hepatocytes. IL-6 is predominately expressed by T cells, macrophages, fibroblasts, endothelial cells and keratinocytes. IL-6 expression can be stimulated by a number of different factors including, T cell mitogens, LPS, viruses, IL-1, TNF, IL-2, IFN-b, platelet derived growth factor (PDGF), protein kinase C, calcium ionophore A23187 and factors that increase the intracellular concentration of cAMP. IL-6 is expressed as a glycoprotein with a variable molecular mass as a result of differential glycosylation/phosphorylation patterns. At least six distinct IL-6 phosphoglycoproteins have been identified. The lower molecular weight species, 23- to 25-kDa, are O-glycosylated while the 28- to 30-kDa species are both O- and N-glycosylated. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible. Purified IL-6 HCX consists of 0-20% carbohydrate by weight.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for IL-6 Biologically Active Protein (NBP1-46086)(1)

Publication using NBP1-46086

• Iankov ID, Penheiter AR, Carlson SK, Galanis E. Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating protein Journal of Immunological Methods 2012-01-01 [PMID: 22750540]

FAQs for IL-6 Biologically Active Protein (NBP1-46086). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6