IL-6 Antibody (5E1)


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Product Details

Reactivity Hu, PmSpecies Glossary
Applications WB, B/N, ELISA, IP
0.1 mg/ml

Order Details

IL-6 Antibody (5E1) Summary

The antibody reacts with human native and recombinant IL-6 as assessed by ELISA.
The antibody reacts with human native and recombinant IL-6 as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant IL-6 as determined with the B9 cell bioassay. The antibody cross reacts with rhesus, cynomolgous natural IL-6.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.2 um filtered antibody solution in PBS, containing and 0.1% bovine serum albumin.
0.02% Sodium Azide
0.1 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Blocking/Neutralizing 50-200 molar excess
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
The antibody is very useful for immuno assays, immunoprecipitation and Western blotting. Furthermore the antibody is useful for neutralization of the biological activity of IL-6.For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions, starting with undiluted material. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-6 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Alternate Names for IL-6 Antibody (5E1)

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta 2
  • IFN-beta-2
  • IL6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A


IL6 is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA (IL6R; MIM 147880), and the shared signaling receptor gp130 (IL6ST; MIM 600694)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-6 Antibody (NBP1-50104). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol IL6

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