IL-6 Antibody (3G9)

Images

 
Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (3G9) [NBP1-47810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL6.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human breast tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human colon tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Kidney tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human lung tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Ovary tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human pancreas tissue using anti-IL6 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IL-6 Antibody (3G9) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 29-212 of human IL-6
Specificity
This antibody is specific for Homo sapiens interleukin 6 (interferon, beta 2) (IL6).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IL6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47810 in the following applications:

Publications
Read Publication using
NBP1-47810 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IL-6 Antibody (3G9)

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta-2
  • IL6
  • IL-6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

IL6 is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA (IL6R; MIM 147880), and the shared signaling receptor gp130 (IL6ST; MIM 600694)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-6 Antibody (NBP1-47810)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Human
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Review for IL-6 Antibody (NBP1-47810) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP1-47810:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IL-6 NBP1-47810
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reviewed by:
Hayley Derricott
IHC-P Human 09/03/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
LotA001
CommentsSecondary antibody applied for 1 hour at RT

Blocking

Blocking DetailsNon-immune block (goat serum, human serum and TBS-T 0.1%) at room temp for 30 mins.

Primary Anitbody

Dilution Ratio10µg/mL, diluted in non-immune block, incubated at 37 degrees for one hour

Secondary Antibody

Secondary DescriptionBiotinylated goat anti-mouse IgG
Secondary Concentration4µg/mL

Details

Detection NotesDetection with DAB, 2-3 minutes
Fixation DetailsFixed in 4% NBF, paraffin wax embedded, heat mediated antigen retrieval in citrate buffer (20 mins)
Wash Description2 x 5 min wash with TBS, 2 x 5 min wash with TBS-T 0.6%

Comments

CommentsSecondary antibody applied for 1 hour at RT

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IL-6 Antibody (NBP1-47810). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IL-6 Antibody (NBP1-47810)

Discover related pathways, diseases and genes to IL-6 Antibody (NBP1-47810). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for IL-6 Antibody (NBP1-47810)

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Blogs on IL-6.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

"Get the skinny" on Adiponectin & Leptin Antibodies
We at Novus Biologicals have an extensive antibody catalog covering lipid and metabolism research, including Adiponectin and leptin antibody products.Excess body fat is linked to many human diseases, including IRS (insulin resistance syndrome), type...  Read full blog post.

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Recent Reviews

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Hayley Derricott
09/03/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol IL6
Entrez