Recombinant Mouse IL-5 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43221
    • Availability
      Product Discontinued

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Recombinant Mouse IL-5 Protein Summary

Description
A Partial Recombinant Proteins to IL-5.
Preparation
Method
Insect cells infected with baculovirus: amino acids Met 21- Gly 133 of mouse IL-5 (accession # NM_010558).
Details of Functionality
The ED50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10^7Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IL5
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
This recombinant mouse IL-5 has been tested by sandwich ELISA using the anti-mouse IL-5 antibody pair (TRFK5/TRFK4), and in bioassays using the IL-5 responsive human cell line TF-1.

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with 0.5% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Mouse IL-5 Protein

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

Recombinant mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL5
Entrez