IL-4 R alpha Overexpression Lysate (Denatured)

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Western Blot: IL4 Receptor alpha Overexpression Lysate (Denatured) [H00003566-T01] - Analysis of IL4 Receptor alpha expression using anti-IL4 Receptor alpha antibody (Cat. # H00003566-B01). Image from verified customer ...read more
IL4R transfected lysate
Lane 1: IL4R transfected lysate (25.6 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IL-4 R alpha Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-IL4R full-length
Gene
IL4R

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 4
using
H00003566-T01 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for IL-4 R alpha

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-IL4R antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IL-4 R alpha Overexpression Lysate (Denatured)

  • CD124 antigen
  • CD124
  • IL-4 R alpha
  • IL-4 receptor subunit alpha
  • IL4R alpha
  • IL-4R subunit alpha
  • IL4R
  • IL-4Ra
  • IL4RACD124
  • IL-4R-alpha
  • interleukin 4 receptor
  • interleukin-4 receptor alpha chain
  • interleukin-4 receptor subunit alpha

Background

Plasmid: pCMV-IL4R Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Review for IL-4 R alpha Lysate (H00003566-T01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00003566-T01:
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Images Ratings Applications Species Date Details
Western Blot IL-4 R alpha H00003566-T01
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reviewed by:
Ming Lu
WB Human 10/03/2014
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Summary

ApplicationWestern Blot
Sample TestedIL4 Receptor alpha Overexpression Lysate (Denatured)
SpeciesHuman
LotE6171
CommentsHere is the issue of "IL4 Receptor alpha Overexpression Lysate (Denatured) Novus Cat#: H00003566-T01, Lot# E6171":I expected a band at 88kDa, but I got the strongest band at ~38kDa. After reading the information of H00003566-T01 and H00003566-B01 (which is the antibody of IL4Ralpha I used), I realized that this overexpress expression is NOT a FULL length, it is actually from NP_001008699, 1 a.a. ~ 227 a.a. But I believe the lysate still works as the positive control for any IL4 Receptor alpha western blot.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution RatioIL4 Receptor alpha Antibody Novus Cat# H00003566-B01 1:1000 overnight 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Novusbio Cat#NB7539
Secondary Concentration1:5000

Details

Detection NotesECL. Got the signal, OK background, exposure of 1 min

Comments

CommentsHere is the issue of "IL4 Receptor alpha Overexpression Lysate (Denatured) Novus Cat#: H00003566-T01, Lot# E6171":I expected a band at 88kDa, but I got the strongest band at ~38kDa. After reading the information of H00003566-T01 and H00003566-B01 (which is the antibody of IL4Ralpha I used), I realized that this overexpress expression is NOT a FULL length, it is actually from NP_001008699, 1 a.a. ~ 227 a.a. But I believe the lysate still works as the positive control for any IL4 Receptor alpha western blot.

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Recent Reviews

4
5
0
4
1
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0
2
0
1
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Ming Lu
10/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol IL4R
Entrez