Recombinant Mouse IL-4 Protein Summary
A partial protein corresponding to IL4.
E. coli expressed amino acids his 21-ser140 of mature mouse IL-4 (accession # NM_021283).
|Details of Functionality
The ED50 measured in a CTLL-2 proliferation assay is typically 100-300 pg/ml, corresponding to a specific activity of approximately 1x10^7-3.3x10^6 Units/mg.
| Protein/Peptide Type
Partial Recombinant Protein
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
This reagent has been tested by sandwich ELISA using the anti-mouse IL-4 antibody pair (11B11/BVD6-24G2) and in bioassays using the mouse cell line CTLL-2.
This protein was made to the mouse sequence.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
20mM Phosphate, 0.38M NaCl, pH 6.5 and 1.0% BSA as a carrier. 0.22 uM filtered.
0.09% Sodium Azide
Purity: Greater than 98%, as determined by SDS-PAGE
Alternate Names for Recombinant Mouse IL-4 Protein
- B cell growth factor 1
- B-cell stimulatory factor 1
- IL-4B_cell stimulatory factor 1
- interleukin 4
- Lymphocyte stimulatory factor 1
Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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