Recombinant Human IL-4 Protein Summary
A partial protein corresponding to IL4.
E. coli expressed amino acids His 25-Ser 153 of mature human IL-4 (accession # AF 395008).
|Details of Functionality
The ED50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10^7Units/mg.
| Protein/Peptide Type
Partial Recombinant Protein
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
This recombinant human IL-4 has been tested by sandwich ELISA using anti-human IL-4 antibody pair (MP4-25D2/8D4-8) and in bioassays using human cell line TF-1.
This protein was made to the human sequence.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 um filtered.
0.09% Sodium Azide
Purity: Greater than 98%, as determined by SDS-PAGE
Alternate Names for Recombinant Human IL-4 Protein
- B cell growth factor 1
- B-cell stimulatory factor 1
- IL-4B_cell stimulatory factor 1
- interleukin 4
- Lymphocyte stimulatory factor 1
Human IL-4 is a ~15-19 kDa factor also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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