Recombinant Mouse IL-4 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42924
    • Availability
      Product Discontinued

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Recombinant Mouse IL-4 Protein Summary

Description
A partial protein corresponding to IL4.
Preparation
Method
E. coli expressed amino acids his 21-ser140 of mature mouse IL-4 (accession # NM_021283).
Details of Functionality
The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 1250 pg/mL. This corresponds to a specific activity of greater than or equal to 8 x 10^5 Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IL4
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
The recombinant mouse IL-4 has been tested by sandwich ELISA using the anti-mouse IL-4 antibody pair (11B11/BVD6-24G2).
Recombinant mouse IL-4 is biologically active and can promote proliferation of mouse T lymphocytes in culture.

Reactivity Notes

This protein was made to the mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Phosphate, 0.38M NaCl, pH 6.5, with no carriers or perservatives. 0.22 um filtered.
Preservative
No Preservative

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Mouse IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL4
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