IL-4 Antibody (MP4-25D2)

Images

 
Western Blot: IL-4 Antibody (MP4-25D2) [NB100-64799] - Analysis of human IL-4 recombinant protein probed with RAT ANTI HUMAN INTERLEUKIN-4 followed by F(ab')2 RABBIT ANTI RAT IgG:HRP (STAR21B).

Product Details

Summary
Product Discontinued
View other related IL-4 Primary Antibodies

Order Details


    • Catalog Number
      NB100-64799
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

IL-4 Antibody (MP4-25D2) Summary

Immunogen
Purified CHO cell expressed recombinant human IL-4.
Specificity
Specific for human Interleukin 4 (IL-4), a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells. Clone MP4-25D2 is reported to neutralise IL-4 activity (2,5).
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
IL4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Positive Control
IL-4 Lysate (NBL1-11957)

Reactivity Notes

Cross reacts with Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-4 Antibody (MP4-25D2)

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of native CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. This clone is reported to neutralise IL4 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NB600-922
Species: Ch, Vi
Applications: WB, ELISA, IHC, IHC-P, IP
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)

Publications for IL-4 Antibody (NB100-64799) (0)

There are no publications for IL-4 Antibody (NB100-64799).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-4 Antibody (NB100-64799) (0)

There are no reviews for IL-4 Antibody (NB100-64799). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-4 Antibody (NB100-64799) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional IL-4 Products

Bioinformatics Tool for IL-4 Antibody (NB100-64799)

Discover related pathways, diseases and genes to IL-4 Antibody (NB100-64799). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-4 Antibody (NB100-64799)

Discover more about diseases related to IL-4 Antibody (NB100-64799).
 

Pathways for IL-4 Antibody (NB100-64799)

View related products by pathway.

PTMs for IL-4 Antibody (NB100-64799)

Learn more about PTMs related to IL-4 Antibody (NB100-64799).
 

Research Areas for IL-4 Antibody (NB100-64799)

Find related products by research area.

Blogs on IL-4

There are no specific blogs for IL-4, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-4 Antibody (MP4-25D2) and receive a gift card or discount.

Bioinformatics

Gene Symbol IL4
Entrez
Uniprot