Recombinant Human IL-33 Protein

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SDS-Page: IL33 Protein [NBC1-18472] - IL-33, 18.1Da (160aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

Order Details

Recombinant Human IL-33 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 112-270 of Human IL-33

Source: E.coli

Amino Acid Sequence: MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 0.1 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL33
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris 8.0
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human IL-33 Protein

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NFHEV
  • NF-HEV
  • NF-HEVNFEHEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2

Background

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. Recombinant human IL-33(C-terminal fragment) was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Blogs on IL-33.

Interleukin 33 (IL-33) - A dual function cytokine
IL-33 is a member of the interleukin family of cytokines that regulates a wide variety of cellular functions. Its receptor is ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and the IL-1R accessory...  Read full blog post.

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Bioinformatics

Gene Symbol IL33
Entrez