IL-33 Antibody [Biotin]

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Western Blot: IL-33 Antibody [Biotin] [NBP1-42758] - Analysis of a band 18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42758
    • Availability
      Product Discontinued

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IL-33 Antibody [Biotin] Summary

Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Specificity
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Clonality
Polyclonal
Host
Rabbit
Gene
IL33
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 18 kDa in size corresponding - mature human IL-32alpha protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Readout System

Reactivity Notes

human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for IL-33 Antibody [Biotin]

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NFHEV
  • NF-HEV
  • NF-HEVNFEHEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2

Background

IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IL-33 Antibody (NBP1-42758)

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Diseases for IL-33 Antibody (NBP1-42758)

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Blogs on IL-33.

Interleukin 33 (IL-33) - A dual function cytokine
IL-33 is a member of the interleukin family of cytokines that regulates a wide variety of cellular functions. Its receptor is ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and the IL-1R accessory...  Read full blog post.

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Bioinformatics

Gene Symbol IL33
Entrez