IL-33 Antibody Summary
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Immunogen affinity purified
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- Western Blot 1:500-1:2000
- ELISA 1:20000-1:100000
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated - be <10 pg/ul by the LAL method. By western blot a band approximately 18 kDa in size corresponding - mature human IL-32alpha protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen affinity purified
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.
Alternate Names for IL-33 Antibody
- C9orf26chromosome 9 open reading frame 26 (NF-HEV)
- DVS27-related protein
- interleukin 33
- Interleukin-1 family member 11
- Nuclear factor from high endothelial venules
IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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