IL-32 Antibody

Images

 
Western Blot: IL-32 Antibody [NBP1-51259] - Analysis of IL-32 (K226) pAb at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264.7 whole cell lysateLane3:H9C2 whole cell lysate
Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-51259] - Analyzes of IL-32 (K226) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: IL32 Antibody [NBP1-51259] - Analyzes of IL32 (K226) pAb in extracts from HepG2 cells.
Immunohistochemistry-Paraffin: IL32 Antibody [NBP1-51259] - Analyzes of IL32 (K226) pAb in paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51259
    • Availability
      Product Discontinued

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IL-32 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 200-2450 of Human IL-32.
Specificity
IL-32 (K226) pAb detects endogenous levels of IL-32 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
IL32
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of IL-32 (K226) pAb in extracts from HepG2 cells.
Immunohistochemistry (IHC) analyzes of IL-32 (K226) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IL-32 Antibody

  • IL32
  • IL-32
  • IL-32alpha
  • IL-32beta
  • interleukin 32
  • interleukin-32 theta
  • interleukin-32
  • natural killer cell transcript 4
  • Natural killer cells protein 4
  • NK4
  • NK4IL-32delta
  • TAIF
  • TAIFa
  • TAIFb
  • TAIFc
  • TAIFd
  • TAIFIL-32gamma
  • Tumor necrosis factor alpha-inducing factor

Background

The interleukins make up a large family of well characterized cytokines, primarily of hematopoietic cell origin. The interleukins are secreted by immune cells (mainly macrophages, B cells and T cells) that regulate a wide range of immune system functions. Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation. IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1beta and IFN-gamma, and by NK cells after exposure to IL-12 and IL-18. It also induces production of IL-1beta, TNFalpha, MIP-2 and IL-6. Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2. The IL-32 protein contains three potential N-myristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites. Human IL-32 exists as four isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL32
Entrez