IL-32 Antibody [Biotin]

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Western Blot: IL-32 Antibody [Biotin] [NBP1-42755] - Detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42755
    • Availability
      Product Discontinued

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IL-32 Antibody [Biotin] Summary

Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.
Specificity
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Clonality
Polyclonal
Host
Rabbit
Gene
IL32
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding - native human IL-32alpha protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Readout System

Reactivity Notes

human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for IL-32 Antibody [Biotin]

  • IL32
  • IL-32
  • IL-32alpha
  • IL-32beta
  • interleukin 32
  • interleukin-32 theta
  • interleukin-32
  • natural killer cell transcript 4
  • Natural killer cells protein 4
  • NK4
  • NK4IL-32delta
  • TAIF
  • TAIFa
  • TAIFb
  • TAIFc
  • TAIFd
  • TAIFIL-32gamma
  • Tumor necrosis factor alpha-inducing factor

Background

IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IL-32 Antibody (NBP1-42755). (Showing 1 - 1 of 1 FAQ).

  1. We would like to detect Human IL-32 gamma isoform. Could you let us know if this antibody can detect IL-32 gamma isoform?
    • NBP1-42755 was raised against a full-length recombinant IL32 protein, which represents the gamma isoform (the other IL32 isoforms are missing amino acids as compared to the gamma). This antibody will recognize the gamma isoform, but because it has not been epitope map I do not know whether it will detect the other isoforms as well.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL32
Entrez