Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] [NBP2-00467] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 ...read more
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] [NBP2-00467] - Human buffy coat. Image verfied by customer.
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation.
Read 1 Review rated 4 using NBP2-00467 in the following application:
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
Protein A or G purified
Alternate Names for IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7]
IL-3 R alpha
IL-3 receptor subunit alpha
IL-3R subunit alpha
interleukin 3 receptor, alpha (low affinity)
interleukin-3 receptor subunit alpha
The 6H6 monoclonal antibody reacts with human CD123, the a chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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