IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7]

Images

 
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] [NBP2-00467] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 ...read more
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] [NBP2-00467] - Human buffy coat. Image verfied by customer.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
6H6
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy7

IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7] Summary

Immunogen
Tissue / cell preparation. IL3R transfected COS cells.
Localization
Type I membrane protein.
Specificity
IL3RA (6H6)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IL3RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-00467 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for IL-3 R alpha/CD123 Antibody (6H6) [PE/Cy7]

  • CD123 antigen
  • CD123
  • hIL3Ra
  • hIL-3Ra
  • IL-3 R alpha
  • IL-3 receptor subunit alpha
  • IL3R alpha
  • IL-3R subunit alpha
  • IL3R
  • IL3RA
  • IL-3Ra
  • IL-3R-alpha
  • IL3RAY
  • IL3RX
  • IL3RY
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin-3 receptor subunit alpha
  • MGC34174

Background

The 6H6 monoclonal antibody reacts with human CD123, the a chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-3 R alpha/CD123 Antibody (NBP2-00467) (0)

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Review for IL-3 R alpha/CD123 Antibody (NBP2-00467) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-00467:
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Images Ratings Applications Species Date Details
Flow Cytometry IL-3 R alpha/CD123 NBP2-00467
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reviewed by:
Anonymous
Flow Human 12/15/2014
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Summary

ApplicationFlow Cytometry
Sample Testedhuman buffy coat
SpeciesHuman

Blocking

Blocking DetailsFC block, 10 min, 4C, 2% human serum, 0.5 mM EDTA in DPBS

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

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FAQs for IL-3 R alpha/CD123 Antibody (NBP2-00467) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

PerCP/Cy5.5 Labeled NBP2-00468

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Bioinformatics Tool for IL-3 R alpha/CD123 Antibody (NBP2-00467)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Anonymous
12/15/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IL3RA
Entrez