IL-3 Antibody


Western Blot: IL-3 Antibody [NBP1-77888] - Analysis using the Biotin conjugate of NBP1-77888. Detection of Lane 1: Human IL-3. Detection of Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at more
Western Blot: IL-3 Antibody [NBP1-77888] - Shows detection of a band ~15 kDa in size corresponding to recombinant human IL-3. The identity of the faint higher molecular weight band may represent a homodimer. Molecular more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

IL-3 Antibody Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None
No Preservative
Please see the vial label for concentration. If unlisted please contact technical services.
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water (or equivalent) to desired concetration.


Application Notes
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated - be <10 pg/ul by the LAL method. By western blot a band approximately 15 kDa in size corresponding - native human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Reactive in human

Alternate Names for IL-3 Antibody

  • Hematopoietic growth factor
  • IL3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGFMGC79399
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor


The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

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Gene Symbol IL3

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