IL-3 Antibody

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Western Blot: IL-3 Antibody [NBP1-77888] - Analysis using the Biotin conjugate of NBP1-77888. Detection of Lane 1: Human IL-3. Detection of Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at ...read more
Western Blot: IL-3 Antibody [NBP1-77888] - Shows detection of a band ~15 kDa in size corresponding to recombinant human IL-3. The identity of the faint higher molecular weight band may represent a homodimer. Molecular ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

IL-3 Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL3
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:10000
Application Notes
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated - be <10 pg/ul by the LAL method. By western blot a band approximately 15 kDa in size corresponding - native human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Reactive in human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water (or equivalent) to desired concetration.

Alternate Names for IL-3 Antibody

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL3
Entrez