IL-27 Partial Recombinant Protein


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Product Details

Reactivity HuSpecies Glossary
Applications IA
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

IL-27 Partial Recombinant Protein Summary

A partial protein corresponding to IL27.
Human EBI3 (met 1-lys229; accession #NM_005755) linked through a (gly-gly-gly-ser)3 to mature P28/IL-30 (phe 29-pro 243; accession # NM_145659) tagged at the C-terminal with thr-gly-his10 was expressed in human 293 cells.
Details of Functionality
This recombinant human IL-27 (EBI3/p28) has been tested in bioassay for inhibition of IL-2 production by mouse splenocytes activated with immobilized anti-CD3 and soluble anti-CD28 antibodies (Villarino, A.V., et al. 2006. J. Immunol. 176: 237). The ED50 for this is typically below 100 ng/ml, corresponding to a specific activity of greater than 1.0 x 10^4U/mg.
Endotoxin Note
Less than 0.01 ng/ug cytokine, as determined by the LAL assay.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Sterile liquid; 20 mM NaHPO4, pH 6.0, 0.6M NaCl, with no carrier or stabilizer. 0.22 uM filtered.
0.09% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.

Reactivity Notes

This protein was made to the human sequence.


Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for IL-27 Partial Recombinant Protein

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A


IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and nave CD4+ T cells mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-gamma-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rbeta2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on nave CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product PDFs


Gene Symbol IL27

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